Runner Up (unintentional): Kirk carrying around 'that' rock on "What Are Little Girls Made Of"
SPOCK: I see. (pause) Gentlemen, I am in command of this vessel, and we shall continue on our present course. (conspiratorial whisper) Unless it is your intention to declare a mutiny.
SCOTT: Mister Spock!
MCCOY: Who said anything about a mutiny, you stubborn, pointed-eared. All right. If we don't find them here, do we still have another search on Gamma Two?
SPOCK: Agreed. Mister Scott, could you manage warp seven?
SCOTT: I would be more than content to do so, sir, and maybe a wee bit more.
SPOCK: Ensign, warp seven.
Nog and Rom scurrying through jeffries tubes only to accidentally crawl into the Sisko's office. Sisko's understated reaction to it just makes things funnier, as if this kind of thing happens all the time.
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