I like DS9 - Rapture. Sisko's brain is all plump with prophecy - he's firing them off two at a time with deadly accuracy... Walking through a throng of fans, he's making ladies swoon and assuring farmers that their crops will return when he spots one guy who rubs him the wrong way and he says to him "You, you don't belong here. Go home." Said reject instantly turns on his heel and slinks away. Reminds me of a couple of parties I crashed in high school.
Kirk:" How many fingers am I holding up." McCoy:"Thats not very damn funny." McCoy:"That hurts worse than the uniform." CCC.
Lots of great ones already mentioned. Here's a couple more: Kirk: The answer is "no". I am therefore going anyway. Worf: With mint frosting. Data trying Guinan's drink in Generations: Data: I hate this! It is revolting! Guinan: More? Data: Please... Most of "Take Me Out To The Holosuite". Big chunks of "Little Green Men". Everyone's reaction to TOS-era Klingons in "Trials And Tribble-ations". Seven Of Nine channeling a Ferengi when she develops multiple personalities.