It was the same look on the Russian guy's face the first time they did the purported joke.
Professor Zoom wrote:
...Facts iz facts...The look on Loki's face when he realizes he's been taken... hilarious.
Don't want to be quarrelsome, but it sure isn't a fact that the same joke is funnier the second time round. I don't think there was even any particular mirth the first time.
Now it's nice for you if you could believe that, but really, since there's no difference between Loki's dumb expression and Russian whozis' dumb expression, the bang from the second scene comes from the pizzazz added by Loki's use of the word "quim."
There's a reason why I rated the movie as no better than a C. And there's a reason why I found it completely unwatchable on DVD. The movie's just not good enough without the real pizzazz of a movie screen. This reliance on shock value in this scene exposes Whedon's failure to write a good script.