For me, it goes to Rom hollering WAAAAAAAAAAAAAAIT in the DS9 season 5 episode Doctor Bashir, I Presume.
That scene just gets on my last good nerve.
Okay, now I wanna hear from you what scene from which Trek series gets on your last good nerve.
Haha, I remember that scene.
There were many scenes with Rom in DS9 & Troi in TNG that were epically unbearable....