There are a few instance where "Miss Uhura" is used, and IIRC it's always from Spock. One is in "The Man Trap," when Spock tells her she made an error in the frequency column and they get into the thing about Vulcan's moon. I can't remember the rest, but I know there are a couple in S1.
Didn't Roger Korby's Kirk android refer to Christine as "Miss Chapel"? Couldn't tell for sure whether he said "Miss", or "Nurse".